Improvement of the Immunomagnetic Separation Method Selective for Escherichia coli O157 Strains
نویسندگان
چکیده
منابع مشابه
Development of an immunomagnetic separation method for efficient enrichment of Escherichia coli O157:H7
Immunomagnetic separation uses antibody-coated paramagnetic particles to selectively bind and purify the target organisms from a comprehensive range of complex food matrices. Aim of this study was to develop and optimize an immunomagnetic separation method based on monoclonal antibody for efficient isolation of Escherichia coli O157:H7 in food samples. The key parameters for preparing the immun...
متن کاملImprovement of immunomagnetic separation for Escherichia coli O157:H7 detection by the PickPen magnetic particle separation device.
Conventional immunomagnetic separation (IMS) procedures, which use an external magnetic source to capture magnetic particles against the side of a test tube, are labor-intensive and can have poor sensitivity for the target organism because of high background microflora that is not effectively washed away during the IMS process. This report compares the conventional IMS procedure to a new IMS pr...
متن کاملCombined use of an immunomagnetic separation method and immunoblotting for the enumeration and isolation of Escherichia coli O157 in wastewaters.
AIMS The detection of Escherichia coli O157:H7 in environmental samples is a human concern. The high persistence of this serotype in the environment suggests that contaminated animal wastewater could act as a potential reservoir. Nevertheless, the high levels of background microflora and cell damage because of environmental stress hamper the isolation of this pathogen without using enrichment m...
متن کاملPrevalence of Escherichia coli O157:H7 on dairy farms in Shiraz, Iran by immunomagnetic separation and multiplex PCR
To identify the reservoirs of shiga toxin-producing Escherichia coli O157, sensitive detection andisolation methods are necessary. The sensitivity of traditional culture methods can be improved significantlyby the inclusion of an immunoconcentration step, resulting in less false-negative results. In this study,enrichment procedure and immunomagnetic separation (IMS) were compared for use in con...
متن کاملImmunomagnetic separation of Escherichia coli O26, O111 and O157 from vegetables.
AIMS Raw fruits and vegetables have been increasingly associated with human infections caused by Shiga toxin-producing Escherichia coli. This study evaluates the isolation and detection of E. coli O26, O111 and O157 from vegetable samples using immunomagnetic particles. METHODS AND RESULTS Standard cultivation and immunomagnetic separation (IMS) procedures were compared. It was found that imm...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1998
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.64.1.376-382.1998